Deficiency in Homogentisic Acid Oxidase Activity Associated with the Brown Phenotype of Dictyostelium discoideum

Homogentisic Acid Oxidase*

In the course of a study of the properties of the homogentisic acid oxidase of rat liver the activity of other animal tissues has been briefly examined. The enzyme has been reported to occur in beef and hog (I), guinea pig (2), and rabbit (3) livers and in a strain of Pseudomonas (4). The experiments reported here show that the enzyme is also present in pigeon liver and in guinea pig, rabbit, p...

Studies on Tyrosinosis: 2, Activity of the Transaminase, Parahydroxy-phenyl-pyruvate Oxidase, and Homogentisic-acid Oxidase.

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Experimental studies of enzyme kinetics in vitro and metabolic fluxes in vivo have been used by Wright and her colleagues to develop a detailed kinetic model of the tricarboxylic acid cycle in Dictyostelium discoideum. This model has recently been been analyzed by two different methods (Albe, K. R., and Wright, B. E. (1992) J. Biol. Chem. 267, 3106-3114; Shiraishi, F., and Savageau, M. A. (199...

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of 02 utilization was also determined over differe...

Control of SCAR activity in Dictyostelium discoideum.

The WASP (Wiskott-Aldrich syndrome protein)/SCAR (suppressor of cAMP receptor) family of adaptor proteins regulate actin polymerization by coupling Rho-family GTPases to the activation of the Arp2/3 complex. SCAR exists within a complex of proteins, including Nap1 (Nck-associated protein 1), PIR121 (p53-inducible mRNA 121), Abi2 (Abl-interactor 2) and HSPC300. This complex was first reported to...